Kawm txog Betulin hauv ob peb feeb.

Betulin yog dab tsi?

Betulin yog SREBP inhibitor nrog IC50 ntawm 14.5 μM hauv K562 hlwb.

Bioactivity

Lub hom phiaj: IC50: 14.5 μM (SREBP, K562 cell), 74.1 μM (SREBP, HeLa cell), 17.1 μM (SREBP, GOTO cell) [1], 21.09 μM (SREBP, 181P cell), 20.62 μM (SREBP, SREBP) )

Cov kev tshawb fawb hauv vitro: Betulin (BE) muaj ntau yam khoom siv roj ntsha thiab tshuaj, ntawm cov tshuaj tiv thaiv kab mob thiab tshuaj tua kab mob tau nyiam feem ntau ntawm cov neeg mob. BE tau pom tias yuav tshem tawm cov khoom tiv thaiv kab mob los ntawm inhibiting qog nqaij hlav qog noj ntshav. Nyob ntawm hom mob qog noj ntshav, BE tau qhia ntau yam ntawm kev tiv thaiv kev tiv thaiv kab mob, xws li tsis muaj zog inhibition ntawm kev loj hlob ntawm tib neeg erythroleukemia cell kab (K562) hlwb kom muaj zog inhibition hauv tib neeg neuroblastoma hlwb (SK-N-AS), qhov twg. feem ntau pom tseeb tshwm sim. Tsis tas li ntawd, BE kuj tau pom los qhia txog cytotoxicity tseem ceeb rau cov kab mob qog noj ntshav uas cais tawm ntawm cov qog qog tau los ntawm cov neeg mob ntawm zes qe menyuam, ncauj tsev menyuam thiab glioblastoma, nrog rau IC50 qhov tseem ceeb ntawm 2.8 txog 3.4 μM, piv rau qis dua qhov ntawd.tsim cov kab ntawm tes. Kev sib piv ntawm crude birch bark extract thiab purified betulin thiab betulinic acid nyob rau hauv tib neeg gastric cancer (EPG85-257) thiab human pancreatic cancer (EPP85-181) tshuaj susceptibility thiab kuj (daunorubicin thiab mitoxantrone) hlwb cytotoxic kev ua ntawm lub kab. tau pom qhov sib txawv tseem ceeb hauv kev nkag siab ntawm cov kab ntawm tes, nyob ntawm qhov sib xyaw siv, qhia tias ob qho tib si betulin thiab betulinic acid tuaj yeem suav hais tias muaj txiaj ntsig zoo rau kev kho mob qog noj ntshav.

Hauv kev tshawb fawb vivo: Betulin tuaj yeem txhim kho cov piam thaj tsis kam rau siab thiab txhim kho kev kawm yooj yim. Kev kho Betulin tuaj yeem rov ua haujlwm hippocampal SOD thiab txo MDA cov ntsiab lus. Betulin kuj txo qis cov qib ntawm inflammatory cytokines hauv cov ntshav thiab hippocampus. Tsis tas li ntawd, kev tswj hwm ntawm BE ua tau zoo tshaj qhov kev qhia ntawm Nrf2, HO-1 thiab thaiv cov phosphorylation ntawm IκB, NF-κB. Hauv kev xaus, BE tuaj yeem muaj kev tiv thaiv ntawm STZ-vim kev paub txog kev poob qis hauv cov nas mob ntshav qab zib los ntawm HO-1/Nrf-2/NF-κB txoj hauv kev

Solubility: Hauv vitro: 10mM hauv DMSO

Kev daws teeb meem Tshuag:

1 mM 2.2588 mL 11.2938 mL 22.5876 mL

5 mM 0.4518 mL 2.2588 mL 4.5175 mL

1 0 mM 0.2259 mL 1.1294 mL 2.2588 mL

Cellular assay: Chemoresistance tau sim siv qhov kev ntsuam xyuas kev loj hlob raws li sulforhodamine B staining. Luv luv, 800 cov cell ntawm ib qhov dej tau muab noob rau hauv 96-zoo daim hlau hauv triplicate. Tom qab 24 teev ntawm kev sib txuas, cov tshuaj tau ntxiv rau hauv cov tshuaj dilution rau 5 hnub ntawm incubation ua ntej SRB staining. Incubation raug txiav tawm los ntawm kev hloov qhov nruab nrab nrog 10 feem pua ​​​​ntawm trichloroacetic acid, tom qab ntawd ntxiv incubation ntawm 4 degree rau 1 teev. Tom qab ntawd, cov phaj raug ntxuav tsib zaug nrog dej thiab stains los ntawm kev ntxiv 100 μL ntawm 0.4 feem pua ​​SRB hauv 1 feem pua ​​​​acetic acid rau 10 min ntawm chav tsev kub. Unbound dye raug tshem tawm los ntawm soaking lub phaj tsib zaug nrog 1 feem pua ​​​​acetic acid. Absorbance tau nyeem ntawm 562 nm tom qab cua ziab thiab rov ua dua cov protein-binding dye hauv 10 mM Tris-HCl (pH=8.0)

Tsiaj kev sim: Cov nas:; Cov nas tau muab faib ua 5 pawg (n=10): pawg tswj, pawg STZ, STZ ntxiv rau betulin (20} mg / kg), pawg STZ ntxiv rau betulin (40 mg /kg) pab pawg. Mob ntshav qab zib tau tshwm sim rau 4 lub lis piam siv STZ (30 mg / kg, ip) yaj hauv citrate tsis (pH 4.4, 0.1 M) siv 1 mL syringe, thaum tswj nas tau txais qhov sib npaug ntawm citrate tsis. Tom qab ntawd, cov nas mob ntshav qab zib tau kho nrog betulin (20 mg / kg, 40 mg / kg) ntxiv rau 4 lub lis piam.

Shipping: chav tsev kub; tej zaum yuav txawv

Lub cev thiab tshuaj muaj zog

Specification

98 feem pua

Peb qhov zoo

1.Kev xa khoom sai.Kev lees paub zoo

2.Our R & D pab neeg yog tsim los ntawm post-doctoral, biological thiab zaub mov kawm tiav nrog ywj siab, R & D muaj peev xwm.

3.Muab cov kev pabcuam customized.OEM kev pabcuam suav nrog: softgel, capsule, ntsiav tshuaj, hnab, granule, thiab lwm yam. Kev pabcuam los tsim cov khoom lag luam tshwj xeeb nrog ntau dua 100 tus neeg siv khoom thoob ntiaj teb.

4.Over 10 xyoo dhau los hauv herballngrents ntau lawm raws li GMPstandard. Tag nrho cov kev paub ntawm tshuaj ntsuab extracts zoo tswj thiab daim ntawv thov.