Part1.Txiv neej noj tshuaj
Gossypol acetate hmoovyog ib qho khoom xyaw uas tau muab rho tawm los ntawm cov noob paub tab lossis cov cag ntoo ntawm Malvaceae cog Upland paj rwb lossis lwm cov nroj tsuag ntawm tib lub genus, thiab tau siv dav hauv kev kho mob hauv daim ntawv ntawm gossypol acetate. Qhov ncauj gossypol acetate tau ua tiav siv rau txiv neej kev tiv thaiv kab mob rau ntau xyoo, thiab muaj kev tiv thaiv qog, tiv thaiv kab mob, tiv thaiv kab mob thiab lwm yam dej num.
Ntu 2. Lub cev thiab tshuaj muaj zog
Nrog rau kev hloov pauv ntawm crystallization tej yam kev mob, gossypol acetate yog koob-zoo li, flake-zoo li, thiab qws-zoo li multi-morphic crystals, nrog luster, tsw, thiab tasteless. Nws yog ib qho yooj yim rau deteriorate thaum raug lub teeb thiab cua sov. Nws muaj solubility siab nyob rau hauv lwm cov kuab tshuaj, 50-200g ib liter, me ntsis soluble nyob rau hauv roj av ether, insoluble nyob rau hauv dej, thiab ua sawv liab thaum reacted nrog concentrated sulfuric acid, tsaus ntsuab thaum reacted nrog 1 feem pua ferric chloride kua, thiab yog incompatible nrog rwb thaiv tsev Cov kev sib txhuam ntawm cov khoom muaj ib tug electrostatic cov tshuaj tiv thaiv, thiab nws melting point nws txawv nrog lub xeem refined crystallization tej yam kev mob, tab sis feem ntau lub melting point yog nyob ib ncig ntawm 174-176 degree .
Ntu 3. Qhov chaw
Cottonseed yog tsim los ntawm cottonseed husk thiab paj rwb kernel (kab mob). Cov paj rwb kernel nyob rau hauv husk yog tsim los ntawm plaub qhov chaw: cotyledon, kab mob, embryo qia, thiab radicle. Muaj ob cotyledons, uas suav rau feem ntau ntawm cov embryo. Nws yog curled thiab folded nyob rau hauv lub plhaub. , lub cotyledon muaj ntau oval daj chromogens, uas suav txog li 1.2 feem pua rau 2 feem pua ntawm qhov hnyav ntawm paj rwb kernels. Cov chromogens muaj ntau yam pigments tsuas yog gossypol. Thaum nws ntsib huab cua Thaum Phau Ntawv Chemical yog kev sib deev hnyav, phab ntsa ntawm cov qog xim yuav tawg thiab tso tawm cov ntsiab lus.
Cov ntsiab lus ntawm gossypol hauv cottonseed yog hais txog {{0}}.15 feem pua -1.8 feem pua . Ntawm lawv, cov ntsiab lus nyob rau hauv paj rwb hull yog 0.005-0.01 feem pua , thiab cov ntsiab lus nyob rau hauv paj rwb kernels yog 0.5-2.5 feem pua . Muaj cov qog gossypol suav txog 1.5-2.7 feem pua ntawm cov txiv ntoo hnyav hauv paj rwb, thiab nws cov xim yog los ntawm lub teeb daj mus rau cherry liab. Nws yog qhov tuab tshaj plaws ntawm gossypol hauv paj rwb, thiab gossypol suav txog li ntawm 35-50 feem pua ntawm cov qog nqaij hlav.
Ntu 4. Txoj kev tsom xam
1) Kev ua haujlwm siab ua kua chromatography: Dej ua haujlwm siab ua kua chromatography (510 twj tso kua mis, U6K injector) Hypersil BDSC185μm150mm × 4.0mmID chromatographic kem, kem kub: 35 degree, kem siab: 1500psi, mobile theem: methanol: dej (nrog acetic acid Kho PH{11}}.0)=80:20(v/v), txaus tus nqi: 0.5ml/min, qauv ntim 5ul, ntes: 991 diode array ntes, wavelength ntau: 230 ~ 300nm.
2) Kev ua haujlwm siab ua kua chromatography: C18 kem (4.6mm × 150mm, 5μm), theem mobile yog methanol thiab 2 feem pua phosphoric acid aqueous tov (90:10 los ntawm ntim), kem kub yog 40 degree, tus nqi ntws yog 1mL / min, thiab nrhiav tau wavelength yog 235nm.
Ntu 5. Pharmacological kev ua
Gossypol muaj pom tseeb inhibitory nyhuv ntawm spermatogenesis. Qhov tseem ceeb ntawm nws qhov kev txiav txim yog thawj zaug cov phev thiab cov spermatid lig cell uas yog rhiab heev rau gossypol, ua raws li cov spermatocytes ntawm txhua qib. Nrog rau qhov nce ntawm koob tshuaj gossypol, qhov kev puas tsuaj rau cov qog ntshav thiab cov nyhuv puas tsuaj yog sib txuas ntxiv, uas tuaj yeem ua rau txhua yam ntawm cov phev hlwb hauv cov tubules seminiferous poob tag nrho, thiab txawm ploj tag, ua rau lub lumen khoob, thiab feem ntau ntawm cov lumens tsuas pom ib txheej ntawm cov hlwb txhawb nqa ntawm lub hauv paus ntawm lub raj phab ntsa thiab ib qho me me ntawm cov spermatogonia. cov hlwb thiab tsis sib haum.
Gossypol acetate muaj cov nyhuv inhibitory ntawm steroid hormone receptors ntawm zes qe menyuam, endometrium thiab cov leeg nqaij txheej, kom cov endometrium thiab nqaij txheej yog pom tseeb thinned, thiab kev coj khaub ncaws yog txo; siab concentration ntawm gossypol tuaj yeem cuam tshuam cov enzymes lossis kev ua haujlwm ntsig txog kev loj hlob ntawm tes Cov nyhuv ntawm cov protein tuaj yeem ua rau cell apoptosis, tab sis nws yuav tsis ua rau cov cell malignant proliferation; gossypol acetate tuaj yeem ua rau sodium ion, potassium ion thiab chloride ion Reabsorption txo, ua rau lub raum tso tawm ntawm cov poov tshuaj.
