1.Siv ntawmandrotec andrographolide 
Androtec ibndrographolide yog NF-κB inhibitor, uas inhibits NF-κB ua kom dhau los ntawm covalent hloov kho ntawm cysteine residue ntawm p50 hauv endothelial hlwb yam tsis muaj kev cuam tshuam rau IκB degradation lossis p50 / p65 nuclear translocation.
2.Andrographolide Biological Kev Ua Haujlwm
Hauv Vitro: Andrographolide (AP) concentration-dependently suppresses receptor activator ntawm nuclear factor kappa B ligand (RANKL)-mediated osteoclast sib txawv thiab pob txha resorption hauv vitro thiab txo cov kev qhia ntawm osteoclast-specific markers. Andrographolide attenuates o los ntawm inhibition ntawm TNF -induced NF-κB ua kom dhau los ntawm covalent hloov kho ntawm txo Cys62 ntawm p50, tsis cuam tshuam rau IκB degradation los yog p50 / p65 nuclear translocation. Andrographolide tseem inhibits ERK / MAPK signaling txoj kev tsis muaj kev cuam tshuam rau p38 lossis JNK signaling. Andrographolide inhibits osteoclast sib txawv ntawm RAW 264.7 hlwb nyob rau hauv ib tug concentration-dependent yam. Andrographolide suppresses osteoclast tsim nyob rau hauv ib tug concentration-dependent yam tsis muaj kev pom tseeb cytotoxic teebmeem, nyob rau hauv ob qho tib si BMMs thiab RAW 264.7 hlwb. Kev kho mob Andrographolide txo qis thaj tsam ntawm pob txha resorption. Tsuas yog kwv yees li 30 feem pua ntawm cov pob txha resorption pom nyob rau hauv pawg tswj tau ua tiav tom qab kev kho mob nrog 2.5 μM Andrographolide. Osteoclastic pob txha resorption yuav luag tag nrho inhibited tom qab kho nrog 10 μM Andrographolide.
Hauv Vivo: Kev kho mob nrog Andrographolide (5 lossis 30 mg / kg) txo cov pob txha poob vim LPS. Ntxiv mus, Andrographolide me ntsis nce BMD thiab cortex thickness piv rau LPS kev kho mob. Kev tshuaj xyuas histological lees paub qhov kev tiv thaiv ntawm Andrographolide ntawm LPS-vim cov pob txha poob. LPS txhaj tshuaj ua rau inflammatory pob txha yaig thiab nce tus naj npawb ntawm TRAP-zoo osteoclasts.
Kinase Assay: Hauv vitro osteoclastogenesis kev soj ntsuam tau ua ntej los tshuaj xyuas cov teebmeem ntawm Andrographolide ntawm kev sib txawv ntawm osteoclast. Cov hlwb pob txha macrophages (BMM) tau npaj. Luv luv, cov hlwb muab rho tawm los ntawm lub femur thiab tibiae ntawm ib tug {{0}}lub lim tiam-laus C57/BL6 nas yog incubated nyob rau hauv tag nrho cell kab lis kev cai media thiab 30 ng/mL M-CSF nyob rau hauv ib tug T-75 cm2 lub raj mis rau kev loj hlob. Thaum hloov qhov nruab nrab, cov hlwb raug ntxuav kom thiaj li deplete residual stromal hlwb. Tom qab ncav cuag 90 feem pua ntawm qhov sib xyaw, cov hlwb raug ntxuav nrog PBS peb zaug thiab trypsinized rau 30 feeb kom sau BMMs. Cells adhering rau hauv qab ntawm lub tais yog cais raws li BMMs; cov BMMs no yog plated nyob rau hauv 96-zoo daim hlau ntawm ib tug ceev ntawm 8 × 103 hlwb ib qhov dej nyob rau hauv triplicate thiab incubated nyob rau hauv ib tug humidified incubator uas muaj 5 feem pua CO2 ntawm 37 degree rau 24 teev. Tom qab ntawd cov hlwb raug kho nrog ntau qhov ntau ntawm Andrographolide (0, 2.5, 5, lossis 10 μM) ntxiv rau M-CSF (30 ng / mL) thiab RANKL (50 ng / mL). Tom qab 5 hnub, cov hlwb raug kho thiab stained rau tartrate-resistant acid phosphatase (TRAP) kev ua. TRAP-positive multinucleated hlwb nrog ntau tshaj tsib nuclei raug suav tias yog osteoclasts.
Cell Assay: Cov teebmeem ntawm Andrographolide ntawm kev loj hlob ntawm tes raug txiav txim nrog CCK{{0}}. BMMs yog plated nyob rau hauv 96-zoo daim hlau ntawm ib tug ceev ntawm 3 × 103 hlwb ib qhov dej nyob rau hauv triplicate. Nees nkaum plaub teev tom qab, cov hlwb raug kho nrog kev nce ntxiv ntawm Andrographolide (0, 2.5, 5, 10 lossis 20 μM) rau 2 hnub. Tom ntej no, 10 μL CCK-8 yog ntxiv rau txhua qhov dej, thiab cov phiajcim tau muab tso rau ntawm 37 degree rau 2 teev ntxiv. Qhov ntsuas qhov muag pom (OD) yog tom qab ntsuas nrog ELX800 absorbance microplate nyeem ntawv ntawm lub wavelength ntawm 450 nm (650 nm siv). Lub cell viability yog xam.
Tsiaj Admin: Cov nas C57BL / 6 nas (8 lub lis piam) muab faib ua plaub pawg ntawm xya nas txhua. Cov nas raug txhaj ip nrog Andrographolide (5 lossis 30 mg / kg lub cev hnyav) lossis PBS raws li kev tswj hwm 1 hnub ua ntej txhaj tshuaj LPS (5 ug / g lub cev hnyav). Andrographolide lossis PBS txhaj tshuaj intraperitoneally txhua hnub rau 8 hnub. LPS txhaj tshuaj intraperitoneally rau hnub ib thiab plaub. Tag nrho cov nas raug tua 8 hnub tom qab thawj zaug txhaj tshuaj LPS, thiab sab laug femurs ntawm tag nrho cov tsiaj raug kuaj nrog lub siab daws teeb meem micro-CT ntawm qhov kev daws teeb meem ntawm 9 μm.
Specification
98 feem pua
Peb qhov zoo
1.Kev xa khoom sai.Kev lees paub zoo
2.Our R & D pab neeg yog tsim los ntawm post-doctoral, biological thiab zaub mov kawm tiav nrog ywj siab, R & D muaj peev xwm.
3.Muab cov kev pabcuam customized.OEM kev pabcuam suav nrog: softgel, capsule, ntsiav tshuaj, hnab, granule, thiab lwm yam. Kev pabcuam los tsim cov khoom lag luam tshwj xeeb nrog ntau dua 100 tus neeg siv khoom thoob ntiaj teb.
4.Over 10 xyoo dhau los hauv herballngrents ntau lawm raws li GMPstandard. Tag nrho cov kev paub ntawm tshuaj ntsuab extracts zoo tswj thiab daim ntawv thov.
